Bethlehem Steel FC
Chicago Fire FC II
Chicago Fire FC II/Columbus Crew 2
Columbus Crew 2
Crown Legacy FC
FC Cincinnati 2
Houston Dynamo 2
Inter Miami CF II
LA Galaxy II
Los Angeles FC II
New York City FC II
North Texas SC
North Texas SC/St. Louis City 2
North Texas SC/Tacoma Defiance
Orlando City B
Philadelphia Union II/Columbus Crew 2
Philadelphia Union II/Crown Legacy FC
St. Louis City 2
St. Louis City 2/The Town FC
Tacoma Defiance
The Town FC
Vancouver Whitecaps II
Winner SF1
Winner SF2